Retrovirus expression vetor containing hEPO gene and transgenic animal cell and poultry thereby

摘要

The present invention relates to a retrovirus (retrovirus) vectors, thereby transformed by for poultry and a method for manufacturing of the gene encoding the erythropoietic stimulating factor in human blood (human erythropoietin) and the transition expression. ; According to the retroviral vector of the present invention to transition the hEPO gene of people efficiently can be effectively transformed to express the hEPO gene to the cells and poultry of different animals, the poultry is also hEPO gene is transferred to the germ line It can establish a variety of transgenic poultry. ; The transformed animal cells or poultry to minimize the physiological side effects can be controlled by the expression of hEPO doxycycline, and it may be mass-produced economically by using the hEPO biological reactor. ; Poultry, transformation, self-replication-deficient retroviruses (replication-defective retrovirus), erythropoiesis stimulating factor of the person