Method for Transformation protocol of basidiomycete fungus, Polyporus brumalis using protoplast isolation

摘要

The present invention more particularly relates to a winter transformation method using plasma separation mushroom umbrella winter in the transformation process of the umbrella mushrooms , separating plasma winter umbrella mushroom mycelium ; Relates to a method of transgenic winter mushroom umbrella using a plasma separation , comprising the step of obtaining a transfected gene is inserted to convert winter mushroom umbrella transformant with the addition of the gene in the winter be transformed protoplast umbrella mushrooms .