Method for Detecting Ligands Using Biosensor Based on Fluorescence Resonance Energy Transfer

摘要

The invention FRET (fluorescence resonance energy transfer; fluorescence resonance energy transfer) phenomenon to apply biosensors to using ligands of the detection method in related to, but more specifically, the biosensor configuration to the ligand binding protein (ligand-binding protein), the specific reversible structure annealing at a critical temperature or more (unfolding temperature) appears and, using the phenomenon, the level of the structure annealing depending on the concentration of the ligand, the ligand in the sample by measuring the FRET of the biosensor under conditions to maintain the critical temperature a method for simply detecting. The process according to the invention can be widely applied to various types of biosensors utilizing the FRET ligand-binding protein with a fluorescent material. ; Biosensor (biosensor), FRET (fluorescence resonance energy transfer; fluorescence resonance energy transfer), a ligand binding protein (ligand-binding protein), liver cell membrane binding proteins (periplasmic-binding protein), fluorescent proteins (fluorescent protein)