PCR Primer for Genotyping the Mouse Lep Gene and the Kit Comprising the Same

摘要

Lep ^ob used as an obese and diabetic animal model Lep^{ob in} mice Provided are a PCR (polymerase chain reaction) primer and a PCR kit including the same, which can perform allele type analysis simply, quickly and accurately. PCR primers of the present invention can more stably distinguish mismatches of single base pairs by additionally inserting mismatched base pairs at the 3′-terminal positions, and have been optimized to perform PCR in one tube. Lep^ob allele of mouse It can be analyzed easily, quickly and accurately, and is particularly useful for analyzing Lep^ob genotype of a large amount of mice.;Allele-specific PCR, genotyping, ob mice