METHOD FOR PRODUCING FRACTIONS OF CELLS FROM E.COLI, having proteolytic activity of the protease and inhibits

摘要

A method for producing fractions of E.coli cells having proteolytic and protease inhibitory activity, including preservation of cells buffered in the presence of 80-90% glycerol, followed by removing cell walls 3% Triton X-100 extraction increasing salt concentrations: 0.14 M, 0 35 M; 2 M NaCl, 6 M guanidine hydrochloride with 0,1% β-mercaptoethanol, and evaluation igibiruyuschey protease proteolytic activity and affinity chromatography on Sepharose 4 B immobilized trypsin or trypsin inhibitor.