METHOD purification and fractionation of humic acids and humic substances

摘要

A method for cleaning and fractioning of humic acids and humic substances, characterized in that for obtaining extracts from the natural object with a maximum content of humic acids or humic substances threefold extraction is carried out by treating a sample with 0.1 M sodium hydroxide solution in 10% dimethylsulfoxide, and the combined extracts were subjected to centrifugation at 5 thousand. about minutes and chromatographic purification using Sephadex LH-20 obtained fractions sorbed on detonation nanodiamonds washed from ca. esey and nesorbirovannyh fragments, desorption is carried out using elution buffer mixture or chelator obtained the desired product was dried at 35 ° C, in a thin layer.