METHOD OF MARKERLESS TRANSGENIC PLANTS OBTAINMENT, WHICH SYNTHESIZE END PRODUCT, AND RECOMBINANT PLASMID FOR ITS REALIZATION

摘要

1. A method for markerless transgenic plants which synthesize the desired product, comprising co-cultivation of explants of plants with a strain of bacteria of the species Agrobacterium tumefaciens, harboring the recombinant plasmid with the target gene product, wherein the recombinant plasmid as a recombinant plasmid using PBM carrying the desired product gene and the free of the selectable marker genes for resistance to antibiotics and herbicides, are placed transformed plant explants on a medium containing no antibiotics, to Ying ciation shoots, the shoots obtained are grown on fresh medium to fertile transgenic plants carrying the target gene and having the features of the initial variety, then direct selection are synthesized by plants of the desired product. ! 2. Method according to claim 1, characterized in that the strain of bacteria of the species Agrobacterium tumefaciens strain using Agrobacterium tumefaciens LBA4404 (pAL4404), containing the recombinant plasmid PBM. ! 3. A method according to claim 1, characterized in that the selection of transformants are after regeneration immunoassay or western blot analysis of plants shoots directly by analyzing extracts of young seedling plants formed on a nutrient medium containing no antibiotics, and expressing the desired gene product. ! 4. A method according to claim 1, characterized in that the recombinant plasmid PBM as the target product gene comprises a gene selected from the group of a gene encoding the surface antigen of hepatitis B virus, a gene encoding a mammalian antimicrobial peptide cecropin P1, the delta-endotoxin gene of Bacillus thuringiensis SryIA (b), a gene of bacterial DNA methyltransferase M.EcoRII. ! 5. The method of claim 1, wherein