METHOD OF DETERMINING BACTERIAL LIPOPOLYSACCHARIDE ANTIGENS IN SOIL

摘要

1. A method for determining bacterial lipopolysaccharide antigens in soil comprising forming a soil suspension, followed by sorption of the bacterial cells and lipopolysaccharide as a part of the resulting suspension mikroosadka holding immunnofermentnogo formed mikroosadka analysis with antibodies to lipopolysaccharide with immunoenzymatic measurement of absorbance of the reaction products, the comparison of the measured absorbance values with the reference values, the presence of bacterial lipopolysaccharide and Tigeneh judged by the magnitude of deviation of the measured values ​​from the reference value at least 10-15%. ! 2. A method for determining bacterial lipopolysaccharide antigens in the soil according to claim 1, characterized in that in the preparation of the soil suspension of soil samples were resuspended in 0.05 molar sodium carbonate buffer solution per 1 part of soil - 35-45 parts of buffer solution, and the resulting suspension titrated successive double dilutions using the aforementioned buffer in the wells of the plate for enzyme immunoassay. ! 3. A method for determining bacterial lipopolysaccharide antigens in the soil according to claim 1, characterized in that during the immunoassay to wells with a soil suspension introduced 100 l of 0.05% aqueous solution of polyethylene glycol having a molecular weight of 15,000-25,000 as ballast blocking compound then the blocking solution was removed, then added to the wells at 50 ul of specific antibody to lipopolysaccharide dissolved in phosphate buffered saline containing 0.02% Tween-20 and