MICROVESICULES OBTAINED FROM RECOMBINANT YEAST WITH HEMOSTATIC ACTIVITY AND THEIR APPLICATION

摘要

1. A microvesicle derived from yeast carrying a tissue factor (TF) containing (i) a yeast membrane and (ii) a tissue factor (TF) protein or a variant thereof having procoagulant activity, where a portion of said tissue factor (TF) protein or a fragment thereof possessing procoagulant activity, is embedded in the specified membrane. ! 2. Obtained from the yeast microvesicle according to claim 1, where the specified protein factor TF or its variant with procoagulant activity, glycosylated. ! 3. Obtained from the yeast microvesicle according to claim 1, wherein said TF factor protein or variant thereof having procoagulant activity is part of a fusion protein, said fusion protein comprising a first region containing TF protein or a fragment thereof having procoagulant activity associated with a second region containing another peptide or protein. ! 4. Obtained from the yeast microvesicle according to claim 2, wherein said TF factor protein or variant thereof having procoagulant activity is part of a fusion protein, said fusion protein containing a first region containing TF protein or a fragment thereof having procoagulant activity associated with a second region containing another peptide or protein. ! 5. Obtained from the yeast microvesicle according to claim 3, where the specified fusion protein contains TF protein or a variant thereof having procoagulant activity, and a label. ! 6. Obtained from the yeast microvesicle according to claim 4, where the specified fusion protein contains TF protein or a variant thereof having procoagulant activity, and a label. ! 7. Obtained from the yeast microvesicle according to claim 1, which contains a mature TF protein, preferably mature b�