MESOPHILIC AND THERMOPHILIC ORGANISMS WITH A KNOCK OF GENES AND WAYS OF THEIR USE

摘要

1. An isolated nucleic acid molecule comprising the nucleotide sequence according to any one of SEQ ID NOs: 1-5, 30-31, and 47-61, or its complementary sequence. ! 2. An isolated nucleic acid molecule comprising a nucleotide sequence that has at least 80% identity with the nucleotide sequence of any one of SEQ ID NOs: 1-5, 30-31, and 47-61, or its complementary sequence. ! 3. An isolated nucleic acid molecule according to claim 2 having at least about 95% identity with the nucleotide sequence of any one of SEQ ID NOs: 1-5, 30-31, and 47-61, or its complementary sequence. ! 4. A genetic construct comprising any of SEQ ID NOs: 1-5, 30-31 and 47-61 operably linked to a promoter expressed in a thermophilic or mesophilic bacterium. ! 5. Recombinant thermophilic or mesophilic bacteria containing the genetic construct according to claim 4. ! 6. A vector containing a nucleic acid molecule according to any one of claims 1-3. ! 7. A host cell containing a nucleic acid molecule according to any one of claims 1-3. ! 8. A genetically modified thermophilic or mesophilic microorganism, where the first native gene is partially, essentially, or completely deleted, suppressed, inactivated or suppressed, and the first native gene encodes the first native enzyme involved in the metabolic production of an organic acid or its salt, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. ! 9. A genetically modified microorganism according to claim 8, wherein said microorganism is in