Mutant adenylate cyclase, the DNA encoding it, bacteria FAMILY ENTEROBACTERIACEAE, containing said DNA, AND METHOD FOR PRODUCING L-AMINO ACIDS

摘要

FIELD: medicine.;SUBSTANCE: what is presented is a mutant adenylate cyclase having an amino acid sequence presented as SEQ ID NO: 2 wherein a residue of L-lysine in a position matched with position 432 in SEQ ID NO: 2 is substituted by a residue of L-glutamine, and a version of mutant adenylate cyclase having an amino acid sequence presented as SEQ ID NO: 2 wherein a residue of L-lysine in a position matched with position 432 in SEQ ID NO: 2 is substituted by a residue of L-glutamine containing a deletion, an insertion, an addition and/or a substitution of one or more amino acid residues and having activity of adenylate cyclase according to a sequence presented in SEQ ID NO: 2. What is presented is DNA coding mutant adenylate cyclase. What is also presented is a bacterium of Enterobacteriaceae family containing DNA coding mutant adenylate cyclase that is a producer of proteinogenic L-amino acid. What is disclosed is a method for producing proteinogenic L-amino acid involving bacterium growth in the nutrient medium containing ethanol and/or glycerol as a primary carbon source, and recovery of said L-amino acid from the culture fluid.;EFFECT: invention allows producing high efficacy L-amino acid.;10 cl, 3 tbl, 10 ex