METHOD FOR DETERMINING lysozyme activity in the tear fluid IN INFANTS

摘要

FIELD: medicine.;SUBSTANCE: tear is taken by sterile disks made of a porous material to form a microbial lawn and to determine lysocyme activity by lysocyme diffusion from the disk into the microbial lawn. It is followed by incubation in a thermostat for at least 24 hours. A zone of microbial growth retardation is determined by measuring a diameter of the zone of microbial growth retardation. The diameter of the zone of microbial growth retardation in healthy infants is 28-30 mm, while the diameter of the zone of microbial growth retardation in infants suffering inflammatory eye diseases is 20-24 mm.;EFFECT: method enables determining lachrymal lysocyme activity in infants, including newborns.;2 ex