METHOD OF NEUTRALISING BACILLUS ANTHRACIS SPORES AND VEGETATIVE CELLS

摘要

FIELD: chemistry; biochemistry.;SUBSTANCE: invention pertains to biotechnology. Disclosed is a method of neutralising Bacillus anthracis spores and vegetative cells, involving treatment of an infected surface with a disinfectant which is an aqueous solution containing a mixture of Bacillus anthracis OZR-1, Bacillus anthracis F-2 and Bacillus anthracis FAUT bacteriophages with the activity ratio (PFU/cm³) between Bacillus anthracis OZR-1, Bacillus anthracis F-2 and Bacillus anthracis FAUT equal to 1 :(0.2-1):(0.1 -1) and a spore germination activator L-alanine.The disinfecting solution is prepared by mixing separately stored L-alanine and a complex of bacteriophages which are separately grown and mixed in the specified ratios. Preferably, an aqueous solution containing a mixture of bacteriophages with a concentration of 10¹⁰-10¹² PFU/l and a spore germination activator with a concentration of 0.05-0.15 mol/l is used. With the aim of receiving a full effect and fixing the result, the infected surface is twice treated at an interval of 60-180 minutes and effective disinfection can be achieved by using a solution based on 10¹¹-10¹² phage particles per 10 m² of infected surface.;EFFECT: invention enables efficient and environmentally safe neutralisation of Bacillus anthracis spores and vegetative cells.;4 cl, 2 ex