RECOMBINANT PLASMID pAg85A-CBD, Escherichia coli pREP4, pAg85A-CBD STRAIN, CHIMERIC PROTEIN Ag85A-CBD AND THEIR APPLICATION

摘要

FIELD: medicine.;SUBSTANCE: synthetic gene Ag85A Mycobacterium tuberculosis optimised for heterologous expression in nonpathogenic laboratory strains. The recombinant plasmid pAg85A-CBD consisting of an artificial bacterial operon of chimeric protein containing a promotor area of an early promotor of bacteriophage T5, chimeric protein gene and transcription terminator, a bacterial operon of beta-lactamase and a bacterial ColEl-type replication initiation site is produced. The invention also involves the Escherichia coli strain producing chimeric protein Ag85A-CBD, and also a method of immobilisation, concentration and cellulose purification of produced protein. Besides, the invention refers to the same recombinant protein Ag85A-CBD and an immunogenic composition containing it and used for tuberculous infection immunity induction.;EFFECT: invention allows preparing the producer strain providing a high production level of resistant immunogenic proteins which can be one-stage produced, immobilised and purified, producing the effective immunogenic compositions for tuberculosis.;7 cl, 2 dwg, 5 ex