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Method and test system for investigating the influence of substances on nerve fibers of projection systems
Method and test system for investigating the influence of substances on nerve fibers of projection systems
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机译:调查物质对投射系统神经纤维影响的方法和测试系统
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摘要
A method for examining the influence of substances on nerve fibers of projection systems comprising: a.) Cultivation i.) At least one first tissue sample isolated from a first region of the central nervous system of an animal, ii.) At least one second tissue sample from a second Region of the central nervous system of an animal was isolated, under cell culture conditions, the tissue samples i.) And ii.) Being applied next to each other on a membrane at the beginning of the culture, b.) Adding the substance to be examined, c.) Determining whether there is any the tissue samples i.) and ii.) have formed fiber webs as well as representation of the fiber webs with imaging methods and d.) evaluation of the fiber properties in the images obtained, step d.) comprising the following sub-steps: d1.) skeletonization and extraction of the fibers, d2 .) Determination of nodes of the extracted fibers and continuous reconstruction of the fiber paths between the nodes d3.) continuous ...
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