one oligochromatographische device and methods for their use

摘要

The present invention relates to methods and devices for detecting one or more analytes in a biological sample, preferably a clean liquid sample. This invention in particular relates to improved rapid tests such as 'dipsticks', 'lateral flow' devices and 'flow-through' devices. The invention in particular relates to oligochromatographic devices (1) that make use of a peptide- or hapten-coupled oligonucleotide and a reagent (5) specifically recognizing said hapten or peptide (8) and a conjugated probe (6) that hybridizes specifically to a target sequence (7). It allows to detect specifically the presence of a polynucleotides directly or after molecular amplification steps with the use of a specific genuine internal control and a chromatographic control.