A method for preparing a microbead suspension for use in an assay for determining the presence of antibodies to HIV in a sample and an assay

摘要

A method for preparing a microbead suspension for use in an assay for detecting antibodies to HIV comprising; acylating an HIV antigen in a citraconic anhydride solution for solubilising thereof, precoating polystyrene microbeads with a protein (e.g. BSA, casein, gelatin), coating the precoated microbeads with the acylated HIV antigen and deacylating the HIV antigen coated onto the microbeads in an acidic buffer. After washing, the deacylated HIV antigen coated microbeads are suspended in a Tris/polyethylene glycol buffer and further diluted to 0.375% using 800 mM Tris/1 % bovine serum albumin of pH of 8.5 with constant stirring. The microbead suspension is maintained at a temperature in the range of 2 {C to 8{C for a minimum of one week for equilibration thereof.