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METHOD FOR SELECTIVE ELECTROFUSION OF AT LEAST TWO FUSION PARTNERS HAVING CELL-LIKE MEMBRANE
METHOD FOR SELECTIVE ELECTROFUSION OF AT LEAST TWO FUSION PARTNERS HAVING CELL-LIKE MEMBRANE
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机译:具有类似细胞膜的至少两种融合伙伴的选择性电融合方法
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摘要
PROBLEM TO BE SOLVED: To provide a method for selective electrofusion of at least two fusion partners having cell-like membranes.SOLUTION: There is disclosed a method for the selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising (A) a step to bring the fusion partners in contact with each other and (B) a step to apply an electrical field of a strength sufficient to attain fusion and highly focused on the fusion partners. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.
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