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METHOD FOR SELECTIVE ELECTROFUSION OF AT LEAST TWO FUSION PARTNERS HAVING CELL-LIKE MEMBRANE

机译:具有类似细胞膜的至少两种融合伙伴的选择性电融合方法

摘要

PROBLEM TO BE SOLVED: To provide a method for selective electrofusion of at least two fusion partners having cell-like membranes.SOLUTION: There is disclosed a method for the selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising (A) a step to bring the fusion partners in contact with each other and (B) a step to apply an electrical field of a strength sufficient to attain fusion and highly focused on the fusion partners. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.
机译:解决的问题:提供一种选择性电融合至少两个具有细胞样膜的融合伴侣的方法。解决方案:公开了一种选择性电融合至少两个具有细胞样膜以及细胞或亚细胞的融合伴侣的方法。尺寸,包括(A)使融合伙伴彼此接触的步骤和(B)施加足以实现融合的强度的电场并高度关注融合伙伴的步骤。融合伴侣独立地选自单细胞,脂质体,蛋白脂质体,合成囊泡,卵细胞,去核卵细胞,处于任何发育阶段的精子细胞和植物原生质体。

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