REAGENT FOR QUANTIATIVE PCR AND QUANTITATIVE PCR METHOD

摘要

PROBLEM TO BE SOLVED: To dramatically improve a detective sensibility in a reagent for a quantiative PCR and a quantitative PCR method corresponding to a fluorescent probe method and an intercalator method.SOLUTION: PCR is performed by a reagent 1 for a quantitative PCR including a Taqman (R) probe unit 10 including a Taqman (R) probe 15 with which a metal particle 20, a fluorescent reporter 21, a quencher 22, and a probe 23 are bounded, and configured such that a fluorescent particle and the quencher are separated at the stage where a DNA polymerase reaction is proceeded to a binding region of the Taqman (R) probe 15. The progress of gene amplification is detected by measuring the fluorescent intensity of the fluorescent reporter 21 amplified by a plasmon enhanced field P generated by irradiating the metal particle 20 with excitation light.