Whole blood preparation for cytometric analysis of cell signaling pathways

摘要

The present invention is directed preservation of intracellular protein epitopes, the method of preparing a biological sample of a protein epitope measurement and enables the detection of signal transduction pathways based on the ability to capture the active state of transient epitope. In order to ensure you to save active state intracellular protein epitopes and other epitopes of signal transduction molecules, the method provided by the present invention allows rapid fixation of biological samples, including red blood cells to. Furthermore, the method of the present invention allow the lysis of red blood cells, whereby a biological sample, for example whole blood, bone marrow aspirates, peritoneal fluid, and useful for the cytometric analysis of samples containing red blood cells and other, the method I want to way. The present invention also provides a method, or to recover the intracellular epitope on the antigen that was so inaccessible crosslinking fixative needed to secure the sample to "unmask". Importantly, the method of the present invention, phosphoric acid in a biological sample taken directly from the patient in order to investigate the characteristics specific to the disease - to allow storing and analyzing the epitope level.