Method for producing melanin synthesis promoting substance, melanin synthesis promoting substance, external preparation for skin, method for producing tyrosinase active substance, tyrosinase active substance, and pharmaceutical

摘要

Disclosed is a novel technique by which melanin synthesis can be enhanced. Specifically disclosed is a method for producing a melanin synthesis enhancer. One embodiment of the method comprises: a drying/pulverization step (S100) in which plant tissues of stevia serving as a starting material are dried and pulverized; a fermentation step (S102) in which the dried stevia powder obtained in the drying/pulverization step (S100) is fermented; an extraction step (S104) in which extraction from the fermented stevia powder (hereinafter referred to as "fermented stevia") is carried out using an aqueous ethanol solution, thereby obtaining a liquid extract; a first fractionation step (S106) in which the liquid extract of the fermented stevia is fractionated, thereby obtaining an ethanol-soluble fraction; and a second fractionation step (S108) in which the ethanol-soluble fraction is further fractionated, thereby obtaining a lipid-soluble fraction.