Methods of measuring protein-protein interactions, sugar and - - sugar using the same, and new sugar-immobilized metal nanoparticle method for recovering the protein from the protein interactant

摘要

Methods of measuring protein-protein interactions, sugar and - - sugar inexpensively and easily even only unlabeled by using the new and sugar-immobilized metal nanoparticle, a stable can be immobilized carbohydrate easily protein interactions and a method of recovering the protein from the body easily. I got a maltose-immobilized gold nanoparticles are bound to the gold nanoparticle ligand complex obtained by combining the linker compound and maltose. By adding serial dilutions of concanavalin A, the maltose-immobilized gold nanoparticles, sugar and ConA and maltose - protein interactions body is generated, the red-purple of the colloidal solution from the maltose-immobilized gold nanoparticles is lost. That is, sugar visually without labeling - it was possible to confirm protein-protein interactions.