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Cell constituents analysis method using these cells and constituents analysis system and chip prep cell constituents minutes

机译:使用这些细胞和成分分析系统的细胞成分分析方法和芯片制备细胞成分分钟

摘要

PPROBLEM TO BE SOLVED: To provide a method for quantitatively determining mRNA and proteins localizing among cells or in the cell without losing their localization space information, and a chip device and system for the same. PSOLUTION: This chip is provided by catching the content of the cell on a substrate plate or an electrode in a shape of two dimensionally projecting the cell. For this purpose, an electrophoretic power is utilized, or the cell is fixed by evaporating the water content of the cell instantaneously. The mRNA fixed on a two dimensional plane is identified by using a labelled probe having a labelling substance capable of hybridizing with the same. PCOPYRIGHT: (C)2007,JPO&INPIT
机译:<要解决的问题:提供一种定量测定细胞间或细胞内mRNA和蛋白质定位而不丢失其定位空间信息的方法,以及用于该方法的芯片装置和系统。

解决方案:该芯片是通过将电池中的内容物捕获到呈二维投影形状的基板或电极上而提供的。为此,利用电泳功率,或者通过瞬时蒸发细胞的水分来固定细胞。通过使用具有能够与之杂交的标记物质的标记探针来鉴定固定在二维平面上的mRNA。

版权:(C)2007,日本特许厅&INPIT

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