Heavily operation iodine to three-dimensional structure decision mannered null protein crystal of production manner of the atomic uses evaporation iodine derivative, manner

摘要

Topic Simplicity and method, collection method of phase information and three-dimensional structure decisive method of protein crystal of being able to produce heavily the atomic derivative of protein crystal with high probability are offered.SolutionsOperating iodine protein crystal, the method of producing heavily the atomic derivative of protein crystal. Operating iodine and the hydrogen peroxide which evaporate protein crystal, it produces the atomic derivative heavily. The atomic by the iodine of the protein crystal which is produced by this method derivative is attached on x-ray diffraction test heavily, heavily the method phase information of the diffraction x-ray from the atomic derivative being collected. The method of deciding three-dimensional structure of protein crystal heavily on the basis of the phase information of the diffraction x-ray from the atomic was collected with this method derivative. Selective figure