Screening method of the anti-influenza drug and crystallization with the RNA polymerase expression system built from influenza virus

摘要

Allowing overexpressed the RNA polymerase of influenza virus, to crystallize the RNA polymerase of influenza virus, and the present invention is effective for anti-influenza drugs that target proteins that are highly conserved among species of influenza virus and an object thereof is to provide a screening method for a substance that can be a component. The present invention includes a polypeptide comprising the amino acid sequence 1-81 of the RNA polymerase PB1 subunit polypeptide comprising the amino acid sequence 239-716 of the RNA polymerase PA subunit of influenza A / PUERTO RICO / 8/1934 H1N1 type complexes including, as well, in the presence of the candidate substance, comprising the step of selecting an agent that inhibits the interaction constitutes the RNA polymerase of influenza virus and α-subunit of β-subunit 1, the active ingredient of the anti-influenza drug I provides a screening method for a substance that may be.