DEVICE AND METHOD FOR LABEL-FREE DETECTION OF DNA HYBRIDIZATION

摘要

A device and method for detecting the hybridization of an unmodified target deoxyribonucleic acid (DNA) molecule including exposing a Raman substrate to the unmodified target DNA molecule, where the unmodified target DNA molecule is a complementary DNA molecule to a thiol-terminated probe DNA molecule covalently linked to the Raman substrate. Also, the thiol-terminated probe DNA molecule includes an adenine analog substituted for adenine. The hybridization of the unmodified target DNA molecule to the thiol-terminated probe DNA molecule is detected by measuring a Raman spectroscopic response of the Raman substrate.