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PHOTOPORPHYRINOGEN OXIDASE HAVING ACTIVITY OF IMPARTING RESISTANCE AGAINST ACIFLUORFEN AND GENE THEREOF
PHOTOPORPHYRINOGEN OXIDASE HAVING ACTIVITY OF IMPARTING RESISTANCE AGAINST ACIFLUORFEN AND GENE THEREOF
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机译:光致卟啉原氧化酶具有抗酰氟作用的活性及其基因
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摘要
Protoporphyrinogen oxidase having an activity of imparting acifluorfen resistance and gene thereof are provided. Cyanobacterium protoporphyrinogen oxidase gene is identified by introducing a protoporphyrinogen oxidase gene of Arabidopsis into cyanobacterium, disrupting a cyanobacterium gene with a transposon, selecting a mutant strain in which protoporphyrinogen oxidase gene is disrupted, identifying the disrupted protoporphyrinogen oxidase gene, and isolating the disrupted protoporphyrinogen oxidase gene. This procedure is effective as a gene isolation technique when a protein derived from other organism species that is homologous to a known protein (e.g., protoporphyrinogen oxidase from cyanobacterium) can not be found in a gene database of the other species.
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