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Use of a Histone Deacetylase Gene OsHDT1 in Enhancing Rice Heterosis
Use of a Histone Deacetylase Gene OsHDT1 in Enhancing Rice Heterosis
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机译:组蛋白脱乙酰基酶基因OsHDT1在增强水稻杂种优势中的应用
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摘要
A histone deacetylase gene OsHDT1 which enhances utilization of heterosis in rice is isolated and cloned, said gene consisting of: 1) the DNA sequence of positions 1-894 in SEQ ID NO:1 in the Sequence listing; or 2) a DNA sequence which encodes the same protein as that encoded by the DNA sequence of 1). The histone deacetylase gene OsHDT1 is associated with enhanced utilization of heterosis in rice. When the transgenic plants having no phenotype were crossed with “Zhenshan 97A”, the F1 overexpression hybrid plants have obviously earlier flowering period than F1 wild-type hybrid plants. Moreover, the RNAi inhibition hybrid plants have a decreased seed setting rate compared to the negative control hybrid plants, while the parent RNAi inhibition plants and negative control plants shows no evident difference in the trait of seed setting rate. Western blotting revealed that the gene is capable of deacetylating histone, mainly at H4K16 site. In addition, there exist significant differences in the level of histone modification among “Minghui 63”, “Zhenshan 97” and “Shanyou63”.
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机译:分离并克隆了组蛋白脱乙酰基酶基因OsHDT1,其增强了水稻中的杂种优势的利用,所述基因由以下组成:1)序列表中SEQ ID NO:1中1-894位的DNA序列;或2)编码与1)的DNA序列编码的蛋白质相同的蛋白质的DNA序列。组蛋白脱乙酰基酶基因OsHDT1与水稻杂种优势的利用提高有关。当没有表型的转基因植物与“珍山97A”杂交时,F1过表达杂种植物的开花期明显早于F1野生型杂种植物。此外,与阴性对照杂种植物相比,RNAi抑制杂种植物的结实率降低,而亲本RNAi抑制植物和阴性对照植物的结实率性状没有明显差异。 Western印迹显示该基因能够使组蛋白去乙酰化,主要在H4K16位点。另外,“明慧63”,“镇山97”和“山油63”的组蛋白修饰水平存在显着差异。
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