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Enzymatic Assay for the Quantitative Determination of Phospholipase A1 or A2 Activity in a Sample
Enzymatic Assay for the Quantitative Determination of Phospholipase A1 or A2 Activity in a Sample
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机译:酶法定量测定样品中的磷脂酶A1或A2活性
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摘要
The invention relates to a new method for measuring a calcium-dependent phospholipase A1 or A2 enzymatic activity in a sample, comprising the following steps: a) contacting said sample with a solid phase coated with a fluorochrome-labelled phospholipase A1 or A2 substrate, wherein the molecular coverage is in the range 8 to 30 fluorochrome-labelled phospholipase A1 or A2 substrate molecules/nm2 in conditions where calcium-dependent phospholipase A1 or A2 enzymatic activity is blocked. b) reading the fluorescence emission overtime c) adding a solution to initiate calcium-dependent phospholipase A1 or A2 enzymatic activity d) reading the fluorescence emission overtime, and kits for carrying out said method.
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