LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID WITH CALIBRATION CHAMBER CONTAINING PROBES DESIGNED TO BE NON-COMPLEMENTARY WITH ANY NUCLEIC ACID SEQUENCES IN THE FLUID

摘要

A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having hybridization chambers containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration chamber containing ECL probes designed to be non-complementary to any nucleic acid sequence in the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores.