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LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO STEM-AND-LOOP PROBES
LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO STEM-AND-LOOP PROBES
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机译:使用同时存在于茎和环问题上的碱基进行PCR扩增来检测核酸序列的杂交的LOC
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摘要
A LOC device having a supporting substrate, primer-linked, stem-and-loop probes, each with a nucleic acid sequence that matches one of a plurality of target nucleic acid sequences, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the fluid prior to hybridization with the FRET probes, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light.
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