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USES FOR LONG POLAR FIMBRIAE GENES OF PATHOGENIC ESCHERICHIA COLI STRAINS
USES FOR LONG POLAR FIMBRIAE GENES OF PATHOGENIC ESCHERICHIA COLI STRAINS
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机译:致病性大肠杆菌菌株的长极细菌基因的用途
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摘要
Provided are methods for identifying lpf genes in pathogenic serotypes of the Enterobacteriaceae family and for differentiating Escherichia coli (E. coli) O157:H7 strains in an isolate using primer pairs specific to lpf gene variants, particularly IpfA1 and/or lpfA 2 genes and amplicon size of the PCR product to identify prototypic Enterobacteriaceae serotypes or more specifically, to differentiate strains of E. coli serotype O157:1-17. Differentiation further requires identifying the E. coli isolate's eae gene variant type which in combination with the IpfA1 and/or lpfA2 variant identification provides unique markers. Also provided are the primer pairs and a kit comprising the same.
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