Fluorescent dyes, methods of synthesis and applications thereof

摘要

The present invention provides a category of cyanine dyes having the following general structural Formula I, wherein X is defined as C(CH₃)₂, O, S or Se; m is an integer from 1 to 18, R₁ and R₂ are each independently chosen from H, C_1-18alkyl, OR₇, C_1-6alkyl-OR₇ or halogen; R₃ is pyrrolyl, imidazolyl, piperidinyl, piperazinyl, morpholinyl, guanidino, NHR₅ or N(R₆)₂; R₄ is C_1-18alkyl, benzyl or (CH₂)_mR₃; R₅ is saturated and/or unsaturated, straight-chained and/or branched-chained C_1-18alkyl, hydroxy alkyl, mercapto-alkyl, amino alkyl, acyl, phenyl, naphthyl or benzyl; R₆ is a C_2-18alkyl; R₇ is H or a C_1-18alkyl; Y⁻ is an anion. The mentioned compounds can be applied to staining biological samples, to the fields of nucleic acids labeling, blood cell analysis, clinical diagnosis, immunological assays and etc.;