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Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
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机译:激光诱导的硅微柱阵列上的激光解吸电离和肽测序
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摘要
The present invention provides a method of producing a laser-patterned silicon surface, especially silicon wafers for use in laser desorption ionization (LDI-MS) (including MALDI-MS and SELDI-MS), devices containing the same, and methods of testing samples employing the same. The surface is prepared by subjecting a silicon substrate to multiple laser shots from a high-power picosecond or femtosecond laser while in a processing environment, e.g., underwater, and generates a remarkable homogenous microcolumn array capable of providing an improved substrate for LDI-MS.
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