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Method for determining the viability of cells in cell cultures

机译:测定细胞培养物中细胞活力的方法

摘要

The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
机译:本发明涉及一种确定细胞培养物中细胞活力的方法,所述方法包括以下步骤:用一种染料着色该样品,该染料可以根据单个细胞的活力而渗透到各个细胞中,确定该细胞的比例。上色程度不同。本发明的特征在于,将有色细胞的细胞悬浮液作为样品在样品容器( 10 )中离心直至样品中存在的固体成分沉淀为压缩细胞。饼( 13 ),以及为了确定比例,压缩细胞饼()的不同颜色部分( 131、132、133 )的体积13 )作为衡量不同生存力细胞比例的指标。

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