Process for obtaining plasma rich in platelets and growth factors for use in orthopedic surgery

摘要

Process for obtaining plasma rich in platelets and growth factors for use in orthopedic surgery.Characterized the process consists of a collection of 60 ml of whole blood from the patient, the access roads, the central venous and jugular, access should be free of serum or any medications, conditioning the blood in glass tubes containing the Anticoagulant Sodium Citrate then shall - Carry out centrifugation manual pipe for five minutes at 2200 rpm.Promoting the separation of red blood cells, which have a molecular weight greater, deposited in the bottom section of the tube, while that in the upper section are the total plasma (PRP + PPP) with platelets and in the central section between red cells and plasma total, there is a f INA and whitish, denominadade mist zone, which contains the white blood cells, especially white blood cells.And the larger platelets, possession of a syringe 20 ml are collected in 2 ml of PPP from the plasma total for each of the tubes in a tube, then dry, there is 1 ml of calcium glucanato a10% together with 1 ml of PPP + 1 ml of PPP, and the autologous thrombin this tube is deposited and dried. A container with water heated to a temperature between 39 - 40 degrees.Where it remains in the water bath conducted for approximately 10 minutes. During this procedure, are sucked in the rest of the plasma (PRP) and the area of mist (white blood cells) that are packaged in syringes 10 ml, and the lower portion in the tube dry plasma remainder, i.e. a platelet rich plasma (PRP).