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Purification Methods for Isolating Purified Vesicular Stomatitis Virus from Cell Culture
Purification Methods for Isolating Purified Vesicular Stomatitis Virus from Cell Culture
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机译:从细胞培养物中分离纯化的水泡性口腔炎病毒的纯化方法
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摘要
A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 m filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 m filter and recovering the VSV in the filtered solution.
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