Sequential analysis of biological samples with bleaching of intermediate-fluorescence signals

摘要

Methods for detecting multiple targets in a biological sample are provided. The methods include (a) contacting the sample with a first probe; (b) physically binding the first probe to a first target; (c) detecting a first signal from the first probe; (d) applying a chemical agent to modify the first signal; (e) contacting the sample with a second probe; (f) physically binding the second probe to a second target; and (g) detecting a second signal from the second probe. An associated kit and device are also provided.