PROCESS AND SYSTEM FOR GENETIC AND EPIGENETIC IN VITRO REPROGRAMMING OF CELLS AND USE OF A REPROGRAMMED CELL IN THE PREPARATION OF A PHARMACEUTICAL COMPOSITION

摘要

The invention relates to an epigenetic and genetic treatment method and system. The invention can be used to rejuvenate an adult cell nucleus without passing through an embryonic stage and to obtain a tissue that is genetically-rejuvenated and autologous in relation to the original nucleus, comprising the temporary introduction of a nucleus into an enucleated oocyte (genetic rejuvenation), followed by oocyte extraction prior to cell division. The treated nucleus can then be introduced into an adult cytoplasm originating from the nucleus. In this way, cell divisions from the rejuvenated nucleus take place in an adult autologous cytoplasm and the resulting cells immediately take the form of adult, differentiated and functional cells. The rejuvenated autologous tissue thus created can then be grafted without rejection to the adult tissue of the organism from the treated nucleus. Throughout the inventive procedure, the treated cytoplasms and nuclei remain permanently in the adult state and no artificial cell differentiation is necessary. In this way, the invention is different from standard deep cloning in which the treated cells must return to the embryonic stage and which involves artificial cell differentiation. In addition, the tissues derived from standard cloning are not immunologically compatible with the original adult tissues from the treated nucleus since the cell division thereof takes place in the oocyte which represents a foreign cytoplasm to the nucleus. Moreover, standard cloning enables the creation of whole organisms unlike the inventive method and system which can only be used to treat a single tissue. The possible applications of the invention include, for example, the repair of necrotic tissues, such as a myocardial infarction, and damaged tissues, such as the retina (RMD), heart failure, renal failure, liver failure and osteoporosis, the treatment of cancers that occur with age, such as prostate cancer, rectal cancer, colon cancer, etc. The anodyne implantation of a small volume of autologous, rejuvenated, still-healthy cells from the affected organ should inhibit the aforementioned cancers and the metastases thereof.