AN IN VITRO METHOD FOR REDUCING EFFECTS OF HETEROPHILIC ANTIBODIES DURING SPECIFIC BINDING REACTION OF A BINDING PAIR

摘要

The invention discloses an in vitro method for reducing effects of heterophilic antibodies, particularly effects due to human anti- mouse antibodies, effects due to rheuma factors, haemoglobin, bilirubin and triglycerides; during a specific binding reaction of a binding pair, wherein a first binding member recognises its complementary second binding member, wherein the method comprises the use of an aqueous solution as medium for the specific binding reaction which aqueous solution is comprising: a) a buffer to control pH ; b) a compound A selected from the group consisting of: (i) a compound defined by the general formula I R1-[[CR2R3]p-O]q-R4, wherein R1, R2, R3, R4, p and and q are as defined in the specification , (ii) polyol, and (iii) saccharide c) a non-ionic detergent.