Transgene expression in avians using oviduct specific promoters and SIN vectors

摘要

Disclosed is methods of producing an exogenous protein in a transgenic avian by introducing an exogenous nucleotide sequence into an avian to create a transgenic avian, wherein said exogenous nucleotide sequence comprises a nucleic acid sequence encoding the exogenous protein operably linked to a promoter contained in a self-inactivating (SIN) vector derived from a retrovirus. The exogenous nucleotide sequence is integrated into the genome of the avian such that the exogenous protein is expressed in an oviduct cell of the avian, secreted into lumen of the oviduct and-deposited in a hard shell egg laid by the avian. The exogenous protein is then isolated from the egg white.