Modulation of neurotrophin activity; scrutiny method

摘要

An in vitro method to screen for a compound that alters the binding of at least one neurotrophin and / or a pro-neurotrophin with a receptor in the family of Vpsp domain receptors a) provide an assay to measure the binding of a neurotrophin and / or a pro-neurotrophin to a receptor in the family of receptors with Vps10p domain, b) add the compound to be evaluated to the assay, and c) determine the amount of a neurotrophin and / or a receptor-linked pro-neurotrophin of the family of receptors with Vps10p domain, and d) compare the amount determined in step c) with an amount measured in the absence of the compound to be evaluated, e) where a difference between the two quantities identifies a compound that alters the binding of neurotrophins and / or pro-neurotrophins to the receptor family of receptors with Vps10p domain.