Preparation of N-alkyl phenothiazine high purity and their use in chemiluminescent assays for measuring peroxidase activity

摘要

synthetic procedure for preparing N-alkyl phenothiazine, wherein the síntesisconsiste in: a. Preparing the anion of phenothiazine, b. Reacting said anion with a cyclic alkylsulfonic ester (sultone) with precipitation of said N-alkyl phenothiazine in crystalline form, in step a. Anion preparation fenotiozina takes place in situ in a solution contienetetrahidrofurano by adding a base and wherein said cyclic alkylsulfonic ester (sultone) I estáseleccionado from 1,3-profane sultone and 1,4-butane sultone.