Method for micropropagation monocot based on continuous totipotent cell cultures

摘要

Method for increasing the growth rate of an embryogenic embryogenic cultivocelular totipotent family monocot grass or plant reed in operacionesa industrial scale, comprising cell mass: (a) culturing an explant of tissue shoot apex of monocotyledonous plant on a primary medium in queel explant had been previously treated with a cold temperature and the primary medium comprises auxin oauxina and cytokinin to produce a totipotent embryogenic cell culture; (b) treating the cell culture embryonic totipotent cold temperature to increase the speed decrease of the mass of totipotent embryonic cell culture compared to control elque treatment with a cold temperature is to last 30 days to 325 days which aTemperature in a range of 4 to 10 ° C ; and (c) maintaining totipotent embryogenic cell culture by further cultivation on primary medium and / or enun secondary medium, whereby a culture of a monocotyledonous plant having unamayor growth rate of the embryogenic cell mass with a velocity occurs multiplication of 5x to 8xen 2x to 3x compared to a control without cold treatment.