Modified enzymes are provided in which at least one amino acid, such asasparagine, leucine, methionine or serine, of an enzyme is replaced with acysteine and the thiol hydrogen is replaced with a substituent group providinga thiol side chain selected from the group consisting of: a) -SR1R2, whereinR1 is an alkyl and R2 is a charged or polar moiety; b) -SR3, wherein R3 is asubstituted or unsubstituted phenyl; c) -SR4, wherein R4 is substituted orunsubstituted cyclohexyl; d) -SR5, wherein R5 is C10-C15 alkyl; and e) -SR6wherein R6 is a C1-6 alkyl. Also, methods of producing the modified enzymesare provided, as well as detergent and feed additives and a composition forthe treatment of a textile. A method for using the modified enzymes in organicsynthesis is additionally provided. Further, modified enzymes having improvedactivity, altered pH profile and/or wash performance are provided.
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