METHODS OF PREPARING LYMPHOCYTES THAT EXPRESS INTERLEUKIN-2 AND THEIR USE IN THE TREATMENT OF CANCER

摘要

A method of preparing autologus T-lymphocytes for re-introduction into apatient having cancer, which method comprises obtaining peripheral bloodmononuclear cells (PBMCs) from a patient immunized with an antigen of thecanncer, stimulating the PBMCs with the antigen of the cancer in vitro,transducing the PBMCs with a retrovial vector, which (a) comprises andexpresses a human interleukin-2 (IL-2) coding sequence operably linked to aretroviral promoter, (b) does not comprise an exogenously introduced gene thatenables phenotypic selection, and (c) comprises a viral envelope thatefficiently transduces CD8+ T-lymphocytes; a method of preparing autologoustumor-infiltrating lymphocytes (TILs) for reintroduction into a patient havingcancer, which method comprises obtaining TILs from a tumor of the patient,optionally immunized with an antigen of the cancer, optionally stimulating theTILs with the antigen of the cancer in vitro, and transducing the TILs withthe aforementioned retroviral vector; compositions comprising cells obtainedin accordance with such methods; and methods of treating a patient havingcancer by administering to the patient cells obtained in accordance with suchmethods or compositions comprising same.