METHODS FOR DIRECT DETECTION OF INDIVIDUAL METHOTREXATE METABOLITES

摘要

The present invention provides a method for determining a level of amethotrexate polyglutamate (MTXPG) in a cellular extract by resolving at leastone MTXPG in a cellular extract obtained from a human undergoing methotrexatetherapy; and detecting at least one resolved MTXPG, thereby determining alevel of the resolved MTXPG. A method of the invention can be useful, forexample, for determining a level of MTXPG3. MTXPG4 or MTXPG5 or fordetermining a level of each methotrexate polyglutamate species (MTXPG1 toMTXPG7) present in the cellular extract.