METHODS FOR PREDICTING AND TREATING A STERILE INFLAMMATION AND DISCRIMINATING BETWEEN STERILE AND INFECTIVE INFLAMMATION

摘要

The present invention provides methods for predicting the likelihood that a subject will develop a sterile inflammation, identifying a subject that has increased propensity to later develop a sterile inflammation, determining whether a subject with tissue damage should be administered an antimicrobial agent or a reduced dosage of an antimicrobial agent, and treating a subject with tissue damage that require the steps of: measuring the amount of microbial nucleic acid and peptide in a sample from the subject, measuring the amount of mitochondrial nucleic acid or peptide in the sample, and comparing the amount of microbial nucleic acid or peptide to the amount of mitochondrial nucleic acid or peptide. In the provided methods, an increased ratio of the amount of mitochondrial nucleic acid or peptide to the amount of microbial nucleic acid or peptide indicates a subject that has an increased likelihood of later developing a sterile inflammation, identifies a subject that has an increased propensity to later develop a sterile inflammation, identifies a subject that should not be administered an antimicrobial agent or administered a reduced dosage of an antimicrobial agent, or identifies a subject that should be administered one or more anti-inflammatory agents and not administering, or administering at a reduced dosage, an antimicrobial agent. Kits containing one or more oligonucleotide primers for amplification of microbial nucleic acid and one or more oligonucleotide primers for amplification of a mitochondrial nucleic acid, and kits containing one or more antibodies that specifically bind to microbial peptides and one or more antibodies that specifically bind to mitochondrial peptides are also provided.