EXPRESSION VECTOR COMPRISING HUMAN INSULIN GENE AND CONSTRUCTION METHODS AND APPLICATIONS THEREOF

摘要

An expression vector comprising human insulin gene and construction methods and applications thereof are provided. The fusion protein expression cassette which consists of peanut oleosin gene - human insulin gene driven by Brassicacampestris oleosin gene promoter is inserted between the restriction enzyme sites Hind III and Sac I of the plant binary expression vector pBI121, and the plant expression vector pBINOI comprising human insulin gene is obtained. Methods for preparing human insulin by using the said expression vector to transform oil sunflower to construct plant bioreactor are also provided. The methods can not only improve the yield of human insulin, but also greatly reduce production costs, and are suitable for industrial production.