Chinese Hamster Ovary (CHO) cell model and methods of using such a model can be utilized to model characteristics of a CHO cell line, for example, product production, growth, and culture characteristics. CHO cell lines are optimized by relating a plurality of reactants to a plurality of reactions from a CHO cell, a plurality of reactions from a CHO cell transcriptome, each of the reactions comprising a reactant identified as a substrate of the reaction, a reactant identified as a product of the reaction and a stoichiometric coefficient relating the substrate and the product; a constraint set for said plurality of reactions for said data structures, and commands for determining at least one flux distribution that minimizes or maximizes an objective function when the constraint set is applied to the data structure, wherein the at least one flux distribution is predictive of a physiological function of the CHO cell.
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